The basic working principle of the ultraviolet spectrophotometer is similar to that of the infrared spectrometer. It uses certain frequency of ultraviolet visible light to irradiate the analyzed organic matter, causing the transition of valence electrons in the molecule, which will be selectively absorbed. A spectrum whose absorption varies with wavelength, reflecting the characteristics of the specimen. In the UV-visible range, For a particular wavelength, the degree of absorption is proportional to the concentration of the component in the sample, so that the measured spectrum can be analysed qualitatively and, depending on the comparison of absorption with a standard sample of known concentration, quantitatively.
Ultraviolet spectrophotometer main uses
Uv-vis spectrometers involve a wavelength range of 0.2–0.8 microns (corresponding wave number 50,000-12500 cm-1), which is widely used in organic chemistry research. Usually used for substance identification, purity check, organic molecular structure research. Quantitatively, it can determine the content of each component in complex compounds and mixtures, as well as the dissociation constant of the substance, the stability constant of the complex, the indication end point in molecular weight identification and microtitration of the substance, as well as the detector in high performance liquid chromatography.
The following substances can be determined
Trace antimony in pure copper without tin, mercury in tea, bismuth in pure aluminum metal materials, bismuth in pure nickel metal materials, bismuth in crude lead, palladium, platinum, iridium in catalyst, zinc in rice flour, arsenic in soda, zinc in rice, manganese, molybdenum in low-alloy steel, copper in groundwater, cadmium, cobalt in geological samples, chromium (VI) and total chromium in electroplating wastewater, Cadmium, bismuth in electrolytic copper, silver in fixing solution, cerium rare earth in monazite, mercury in waste mercury hydride catalyst extract, cerium rare earth in wastewater, total cerium rare earth in gold, cadmium, copper, steel, vanadium, cobalt, aluminum, molybdenum, niobium, titanium, tin, yttrium, total rare earth, tantalum in superalloy, Vanadium, cadmium, mercury, cobalt in industrial wastewater, Thorium in nickel, grain waste solution, tin in canned food, barium in silicon barium inoculant, thorium in silicon magnesium alloy, palladium in precious metal secondary alloy, bismuth in copper sample,Cobalt in synthetic ammonia catalyst, molybdenum in alloy steel, total amount of rare earth, molybdenum in nickel, alloy cast iron, mercury in river water, zinc in nutrients of black rice soup, cadmium in environmental water samples, NO2- in environmental water, antimony in brass, yttrium in mixed rare earth oxides, nickel in tungsten, zinc in zinc, cerium rare earth in ores, gallium, scandium, thorium, yttrium in ores, total rare earth, heavy rare earth, lead in clinical samples, Iron in zinc, magnesite, rare earth cerium in aluminum alloy, vanadium, magnesium, strontium, titanium, iron, copper, zinc, germanium, cadmium, potassium chlorate IO3-, thallium in coal ash, cerium in magnesium alloy, calcium in antimony and manganese ore, copper in flour, cerium in molybdenum titanium-zirconium alloy, urine protein, and cerium in nickel-based alloy, Yttrium, Palladium in nickel-aluminum catalyst, lead in beer, zinc in glucose, iron naphthenate in oil, bismuth in lead alloy, lead in lead copper alloy, cadmium, indium in lead mining sample, antimony in lead zinc ore, germanium, bismuth in human hair, cadmium, copper, Zinc, lead removal from container surface, calcium from sinter, cerium from biological samples, aluminum in gypsum, aluminum in limestone, aluminum in quartz sand, lead removal from food packaging materials, molybdenum, germanium from food, nickel from edible oil, calcium from raw cement,Calcium, thallium, copper, zinc, cadmium, barium in barium titanate sintered material, manganese in carbon steel, lead in peach leaves, barium in ceramic capacitor barium titanate sintered material, rare earth cerium in iron alloy, calcium in iron ore, bismuth in copper alloy, lead, cerium and rare earth cerium, bismuth in tin, cadmium, copper sample, germanium in copper ore, lead in soil sample, Rare earth elements of cerium group, thorium in thorium tungsten alloy, thorium in tungsten alloy, cerium in tungsten-cerium alloy, calcium in sodium tungstate, scandium in tungsten products, tellurium in selenium, tantalum in rare earth metals, tin in tin surface copper, indium in tin foil, bisth in tin alloy, tin in tin bronze, arsenic in sulfur washing wastewater, cerium in color tube glass, IO3- in sodium nitrate, lead in wheat flour, zinc, aluminum in zinc alloy, cadmium, Antimony, calcium in serum, thallium in tobacco leaf, calcium in rocks and minerals, cadmium in rock samples, germanium in anode mud, boron in magnesium oxide, cerium in yttrium oxide, bismuth in medicine, bismuth in copper, vanadium, manganese, lead and cadmium in tap water.
Uv spectrophotometer selection: When we choose a variety of instruments, there are certain standards, such as measurement accuracy, or measurement range. When choosing an ultraviolet spectrophotometer, we consider the optical configuration, spectral range, sample type and analytical tool.
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